Restriction Enzymes
New England Biolabs, Inc. SspI-HF – 5000 units
A high fidelity restriction endonuclease that recognizes the sequence AAT_^ATT. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. PstI-HF – 10000 units
A high fidelity restriction endonuclease that recognizes the sequence C_TGCA^G. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. SphI-HF – 2500 units
A high fidelity restriction endonuclease that recognizes the sequence GCATG^C. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes. Examples of nuclease contamination studies for some of our HF restriction enzymes are shown below.
New England Biolabs, Inc. BstAPI – 1000 units
A restriction endonuclease that recognizes the sequence GCAN_NNN^NTGC.
New England Biolabs, Inc. Nb.BsrDI – 1000 units
A restriction endonuclease that recognizes the sequence GCAATG_NN.
New England Biolabs, Inc. AvrII – 100 units
A restriction endonuclease that recognizes the sequence C^CTAG_G.
New England Biolabs, Inc. DpnI – 5000 units
A restriction endonuclease that recognizes the sequence GA_^TC.
- DpnI cleaves only when its recognition site is methylated
- Cleavage of mammalian genomic DNA is blocked by overlapping CpG methylation
- Methylation-sensitive restriction enzyme
- Time-Saver qualified for digestion in 5-15 minutes
- 100% activity in rCutSmart Buffer (over 210 enzymes are available in the same buffer)
New England Biolabs, Inc. NdeI – 4000 units
A restriction endonuclease that recognizes the sequence CA^TA_TG.
New England Biolabs, Inc. XhoI – 25000 units
A restriction endonuclease that recognizes the sequence C^TCGA_G.
New England Biolabs, Inc. MspI – 5000 units
A restriction endonuclease that recognizes the sequence C^CG_G.
New England Biolabs, Inc. BsmI – 500 units
A restriction endonuclease that recognizes the sequence GAATG_CN^.
New England Biolabs, Inc. SbfI-HF – 2500 units
A high fidelity restriction endonuclease that recognizes the sequence CC_TGCA^GG. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes. Examples of nuclease contamination studies for some of our HF restriction enzymes are shown below.
New England Biolabs, Inc. SapI – 250 units
A restriction endonuclease that recognizes the sequence GCTCTTCN^NNN_.
New England Biolabs, Inc. MseI – 500 units
A restriction endonuclease that recognizes the sequence T^TA_A.
New England Biolabs, Inc. XmnI – 1000 units
A restriction endonuclease that recognizes the sequence GAANN_^NNTTC.