Restriction Enzymes
New England Biolabs, Inc. EcoRI-HF – 50000 units
A high fidelity restriction endonuclease that recognizes the sequence G^AATT_C. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. Esp3I – 300 units
A Type IIS restriction endonuclease that recognizes the sequence CGTCTCN^NNNN_....
Cayman Chemical COX-1humnrecombInt 5kU
Source: Active recombinant human His-tagged COX-1 expressed in insect cells • Amino acids: 24-599 • MW: ~70 kDa
New England Biolabs, Inc. NarI – 2500 units
A restriction endonuclease that recognizes the sequence GG^CG_CC.
Apexbio Technology LLC Dpn I(Synonyms: DpnI, DpnI Restriction Enzyme, Dam methylation-sensitive restriction enzyme DpnI), 500U.
Dpn I is a restriction endonuclease sensitive to Dam methylation specifically recognizing and cleaving the adenine N6-methylated sequence GA/TC within DNA Cleavage is dependent on adenine methylation status mammalian CpG methylation overlapping Dpn I sites blocks cleavage and methylation by Dcm is not recognized by this enzyme It is routinely used for the rapid digestion of plasmid DNA PCR fragments and genomic DNA and finds broad utility in genetic typing molecular cloning Southern blotting SNP genotyping and restriction fragment length polymorphism (RFLP) analyses
New England Biolabs, Inc. TaqI-v2 – 20000 units
A restriction endonuclease that recognizes the sequence T^CG_A.
New England Biolabs, Inc. BstBI – 2500 units
A restriction endonuclease that recognizes the sequence TT^CG_AA.
New England Biolabs, Inc. RNase Inhibitor, Human Placenta – 2000 units
RNase Inhibitor, Human Placenta is a recombinant human placental protein which specifically inhibits ribonucleases (RNases) A, B and C. It is not effective against RNase 1, RNase T1, S1 Nuclease, RNase H or RNase from Aspergillus. In addition, no inhibition of polymerase activity is observed when RNase Inhibitor, Human Placenta is used with Taq DNA Polymerase, AMV or M-MuLV Reverse Transcriptases, or Phage RNA Polymerases (SP6, T7, or T3). The 50 kDa protein inhibits RNases by binding noncovalently in a 1:1 ratio with an association constant greater than 1014.
New England Biolabs, Inc. MspJI – 200 units
A restriction endonuclease that recognizes the sequence CNNRNNNNNNNNN^NNNN_.
New England Biolabs, Inc. BtsI-v2 – 2500 units
BtsI-v2 is an engineered and improved version of BtsI that recognizes the sequence GCAGTGNN^.
New England Biolabs, Inc. BamHI – 10000 units
A restriction endonuclease that recognizes the sequence G^GATC_C.
New England Biolabs, Inc. Sau3AI – 1000 units
A restriction endonuclease that recognizes the sequence ^GATC_.
New England Biolabs, Inc. BslI – 1000 units
A restriction endonuclease that recognizes the sequence CCNN_NNN^NNGG.
New England Biolabs, Inc. MlyI – 5000 units
A restriction endonuclease that recognizes the sequence GAGTCNNNNN_^.